Disruption of lig4 improves gene targeting efficiency in the oleaginous fungus Mortierella alpina 1S-4
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منابع مشابه
Disruption of lig4 improves gene targeting efficiency in the oleaginous fungus Mortierella alpina 1S-4.
The oil-producing zygomycete Mortierella alpina 1S-4 is known to accumulate beneficial polyunsaturated fatty acids. We identified the lig4 gene that encodes for a DNA ligase 4 homolog, which functions to repair double strand breaks by non-homologous end joining. We disrupted the lig4 gene to improve the gene targeting efficiency in M. alpina. The M. alpina 1S-4 Δlig4 strains showed no defect in...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2015
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2015.05.020